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Earlham Institute | Decoding Living Systems

We are a genomics, bioinformatics and molecular biology institute focused on tackling global challenges through life science research.
Categories: Education/Reference, Education
Topics: animals, bioinformatics, biology, computational, decoding living systems, earlham institute, engineering, genetics, genomics, microbes, plants, synthetic

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Reference in qPCR www.Gene-Quantification.info
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home
The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve.
The method is based on the fit of Richards' equation to real-time PCR data by nonlinear
regression in order to obtain the best fit estimators of reaction parameters. Subsequently,
these parameters were used to calculate the Cy0 value that minimizes the dependence of
its value on PCR kinetic. Our results
demonstrated that when PCR efficiency was slightly decreased, diluting
amplification mix quantity or adding a biological inhibitor such as IgG, the SCF, Ct
and Cp methods were markedly impaired while the Cy0 method gave significantly more
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