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Phosphatome.Net

A resource on protein phosphatases, a key class of regulatory proteins. Includes genomic and evolutionary analyses (phosphatomes), classification, disease associationa and an extensive database of protein phosphatase genes.
Categories: Education/Reference, Information Technology
Topics: kinase, kinases, kinome, lipid phosphatase, lipid phosphatases, phosphatase, phosphatases, phosphatome, phosphatomes, protein phosphatase

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gene-quantification.eu

gene-quantification.eu gene-quantification.eu           
Reference in qPCR www.Gene-Quantification.info
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analysis using quantitative real-time PCR or RT-PCR (qPCR or
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qpcrupdate.info

qpcrupdate.info qpcrupdate.info           
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analysis using quantitative real-time PCR or RT-PCR (qPCR or
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gmo-qpcr-analysis.com

gmo-qpcr-analysis.com gmo-qpcr-analysis.com           
Reference in qPCR www.Gene-Quantification.info
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technical aspects involved in quantitative gene expression
analysis using quantitative real-time PCR or RT-PCR (qPCR or
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gene-quantification.net

gene-quantification.net gene-quantification.net           
Reference in qPCR www.Gene-Quantification.info
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information platform for qPCR - Main focus of the
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technical aspects involved in quantitative gene expression
analysis using quantitative real-time PCR or RT-PCR (qPCR or
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Reference in qPCR www.Gene-Quantification.info
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information platform for qPCR - Main focus of the
www.GENE-QUANTIFICATION.info page is to describe and summarize all
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gene-quantification.com

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Reference in qPCR www.Gene-Quantification.info
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information platform for qPCR - Main focus of the
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gene-quantification.info

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qpcrupdate.com

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home
The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve.
The method is based on the fit of Richards' equation to real-time PCR data by nonlinear
regression in order to obtain the best fit estimators of reaction parameters. Subsequently,
these parameters were used to calculate the Cy0 value that minimizes the dependence of
its value on PCR kinetic. Our results
demonstrated that when PCR efficiency was slightly decreased, diluting
amplification mix quantity or adding a biological inhibitor such as IgG, the SCF, Ct
and Cp methods were markedly impaired while the Cy0 method gave significantly more
accurate and precise quantification results.

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glyconavi.org glyconavi.org           
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