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PrimerSuite | PrimerDimer

Primer Dimer- program for predicting primer dimer formation

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Topics: bisulfite, bisulfite-converted, design, dimer, dna, genomic, methylation, native, prediction, primer, primerdimer, primersuite

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Reference in qPCR www.Gene-Quantification.info
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Reference in qPCR www.Gene-Quantification.info
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Reference in qPCR www.Gene-Quantification.info
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analysis using quantitative real-time PCR or RT-PCR (qPCR or
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analysis using quantitative real-time PCR or RT-PCR (qPCR or
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The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve.
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regression in order to obtain the best fit estimators of reaction parameters. Subsequently,
these parameters were used to calculate the Cy0 value that minimizes the dependence of
its value on PCR kinetic. Our results
demonstrated that when PCR efficiency was slightly decreased, diluting
amplification mix quantity or adding a biological inhibitor such as IgG, the SCF, Ct
and Cp methods were markedly impaired while the Cy0 method gave significantly more
accurate and precise quantification results.


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